N9011 Proteinase K Solution（20 mg per mL）

Product Introduction

Molecular Biology Grade，For Research Use Only

Cat No. N9011

Size: 1 ml

Concentration: 20 mg/ml (20 μg/ul)

Specific Activity: ≥30 U/mg

Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. The Proteinase K is classified as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

Source

From yeast cells with cloned gene encoding genetically engineered Engyodontium album (Tritirachium album) endolytic protease.

Molecular Weight

29.3 kDa monomer.

Definition of Activity Unit

One unit of the enzyme liberates Folin-positive amino acids and peptides corresponding to 1 µmol tyrosine in 1 min at 37°C , pH 7.5 using denatured hemoglobin as substrate.

Enzyme activity is assayed in the following mixture: 0.08 M potassium phosphate (pH 7.5), 5 M urea, 4 mM NaCl, 3 mM CaCl2 and 16.7 mg/ml hemoglobin.

Application

• Isolation of genomic DNA from cultured cells and tissues

• Removal of DNases and RNases when isolating DNA and RNA from tissues or cell lines

• Determination of enzyme localization

• Improving cloning efficiency of PCR products

Features

• Ready-to-use solution

• Recombinant proteinase K

• Active in a wide range of reaction products 

Components

Storage Condition

-20ºC recommended. The shelf life of proteinase K solution is one year when stored sealed below 4ºC. Proteinase K solution is shipped on Bio ice packs or at room temperature.