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K001 Fast DNA Library Prep Kit

Products & Applications > NGS Library Prep Kit > K001 Fast DNA Library Prep Kit

K001 Fast DNA Library Prep Kit

Price: $600.00~2123.00
K001-A(24 rxns) K001-B(96 rxns)

Product Introduction

Aiming at Illumina high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines end repair and A-Tailing into one step, and reagents are highly premixed, greatly shortening the time of library construction and reducing the error caused by tedious steps. The end preparation, adapter ligation, amplification and purification of the fragmented double-stranded DNA can be performed within about 2 hours. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.

Application

NGS library preparation for Illumina platform

Features

1 Wide sample compatibility

2 High efficiency in library construction

3 Compatible with PCR-free workflow

4 End Repair & A-Tailing Enzyme Mix and Buffer are provided as one tube

Experimental Cases

1. High conversion rate of library.

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Figure 1 Library conversion rate when DNA input was 60ng.

 

2. Whether before or after amplification, compared with other manufacturers, the amplification is very superior.

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Figure 2 The concentration of unamplified library obtained when the DNA sample was 100ng and 1ng, and the end repair and adapter ligation were performed with the library construction kits of different manufacturers.

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Figure 3 The total amount of library obtained after amplified with the same cycles when the DNA sample was 100ng, 60ng, and 20ng, and the end repair and adapter ligation were performed with the library construction kits of different manufacturers.

 

Components

Component

K001-A

(24 rxns)

K001-B

(96 rxns)

End Repair & A-Tailing Mix

480 μl

4×480 μl

Fast DNA Ligase

120 μl

2×240 μl

Fast Ligation Buffer

600 μl

4×600 μl

2× HIFI Library PCR Master Mix

600 μl

4×600 μl

Primer Mix

120 μl

480 μl

Storage Condition

All reagents should be stored at -20°C. 


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