N9012 Proteinase K Solution（100 mg per mL）

Product Introduction

Molecular Biology Grade，For Research Use Only

Cat No. N9012

Size: 1.6 L

Concentration: 100 mg/ml

Specific Activity: 180 U/mg

Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. The Proteinase K is classified as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

Source

From yeast cells with cloned gene encoding genetically engineered Engyodontium album (Tritirachium album) endolytic protease.

Molecular Weight

29.3 kDa monomer.

Definition of Activity Unit

One unit of the enzyme liberates Folin-positive amino acids and peptides corresponding to 1 µmol tyrosine in 1 min at 37°C , pH 7.5 using denatured hemoglobin as substrate.

Enzyme activity is assayed in the following mixture: 0.08 M potassium phosphate (pH 7.5), 5 M urea, 4 mM NaCl, 3 mM CaCl2 and 16.7 mg/ml hemoglobin.

Application

• Isolation of genomic DNA from cultured cells and tissues

• Removal of DNases and RNases when isolating DNA and RNA from tissues or cell lines

• Determination of enzyme localization

• Improving cloning efficiency of PCR products

Features

• Concentrated solution

• Ready-to-use solution

• Recombinant proteinase K

• Active in a wide range of reaction products 

Components

Storage Condition

-20ºC is recommended. The shelf life is 5 years when stored below -20ºC.

Ship with Bio ice packs or at room temperature.