R1021 TRAzol Reagent

Product Introduction

TRAzol Reagent is a ready-to-use reagent for the isolation of total RNA from cells and tissues. The reagent, a mono-phasic solution of phenol and guanidine isothiocyanate, is an improvement to the single-step RNA isolation method. During sample homogenization or lysis, TRAzol Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components. Addition of chloroform followed by centrifugation, separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase, the RNA is recovered by precipitation with isopropyl alcohol. After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation. Precipitation with ethanol yields DNA from the interphase, and an additional precipitation with isopropyl alcohol yields proteins from the organic phase.

Copurification of the DNA may be useful for normalizing RNA yields from sample to sample.This technique performs well with small quantities of tissue (50-100 mg) and cells (5×106), and large quantities of tissue (≥1 g) and cells (>107) of human, animal, plant, or bacterial origin.The simplicity of the TRAzol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA isolated by TRAzol Reagent is free of protein and DNA contamination.   

Application

Purified RNA is free of impurities and enzyme inhibitors, and have an A260/280=1.8-2.0, is suitable for applications such as:

•Reverse transcription PCR (RT-PCR)

•Real-time quantitative PCR (qPCR)

•Northern blotting

•Nuclease protection assays

•RNA amplification for microarray analysis

•cDNA library preparation after poly(A)+ selection

Features

•Stable yield

•Reliable performance of high-quality purified total RNA in downstream applications

Components

Storage Condition

Store at 2-8°C, protect from light, and is stable for up to 12 months when stored properly.