R1051 General RNA Extraction Kit

Product Introduction

General RNA Extraction Kit provides a simple method of isolating total RNA from a wide range of sample types and amounts. In general, samples are lysed and then homogenized in the presence of guanidinium isothiocyanate, a chaotropic salt which is capable of protecting the RNA from endogenous RNases. After homogenization, ethanol is added to the sample. The sample is then processed through a spin column containing a clear silica-based membrane where the RNA binds. Any impurities are effectively removed by subsequent washing. The purified total RNA is then eluted in RNase-Free Water and is suitable for use in a variety of downstream applications.  

Application

Purified RNA is free of impurities and enzyme inhibitors, and have an A260/280=1.8-2.0, is suitable for applications such as:

•Reverse transcription PCR (RT-PCR)

•Real-time quantitative PCR (qPCR)

•Northern blotting

•Nuclease protection assays

•RNA amplification for microarray analysis

•cDNA library preparation after poly(A)+ selection

Features

•Stable yield

•Reliable performance of high-quality purified total RNA in downstream applications

Components

Storage Condition

Solution RL store at 2-8°C, protect from light. Other reagents store at room temperature (RT, 15-25°C). And transport at RT. Kit contents are stable for up to 12 months when stored properly.