Product Introduction
The kit provides a convenient and fast DNA Illumina library construction solution for short sequence DNA samples such as PCR products and plasmids, which requires only three simple steps, greatly reducing library construction time and errors caused by troublesome steps. The library can be built quickly for PCR products or plasmids of 150-30,000 bp without primer synthesis, and the DNA can be multiplexed PCR amplification products.
Application
• NGS library preparation for Illumina platform
Features
1 Short time for library construction
2 Easy to operate
3 No need to design and synthesize primers
Experimental Cases
Library Preparation Process
Components
Component | K009-A (24 rxns) | K009-B (96 rxns) |
ShortSeq Mix | 450 μl | 2 × 960 μl |
ShortSeq Buffer B | 50 μl | 400 μl |
2× HIFI Library PCR Master Mix | 240 μl | 960 μl |
Primer Mix | 24 μl | 96 μl |
Storage Condition
All reagents should be stored at -20°C.