Plant DNA Extraction Kit
(Silicone Membrane Centrifugal Column Method)
Cat. No./Spec.: N1221/24 preps; N1222/100 preps
Kit Contents
Component | N1221 | N1222 |
Buffer PAL | 20 ml | 80 ml |
Buffer BDP | 20 ml | 80 ml |
Buffer GWP | 20 ml | 110 ml |
Buffer SW2 | 30 ml | 110 ml |
Elution Buffer | 10 ml | 20 ml |
DNA Spin Column | 24 pcs | 100 pcs |
Collection Tube | 24 pcs | 100 pcs |
Description
This product combines the CTAB extraction method with column purification technology, suitable for extracting high-purity DNA from various plants and fungal samples. The obtained DNA can be directly used for PCR, SSR, AFLP, RAPD, and Southern blot experiments. The kit provides a less toxic substitute for chloroform (Buffer BDP) and uses an alcohol-free, high-concentration guanidine salt-mediated filtration purification to efficiently remove polysaccharides, polyphenols, and other metabolites and RNA from the samples.
Storage
This product can be stored at room temperature (15~25 ̊C) for 18 months. Buffer PAL may precipitate at low temperatures, which can be dissolved by incubating at 55ºC and mixing thoroughly. The product uses PET reagent bottles, which should not be subjected to high-temperature, high-pressure sterilization or direct incubation above 55ºC.