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V5005H DSPath 4X One-Step Multiplex Master Mix (High ROX+)

Products & Applications > 1-step RT-qPCR > V5005H DSPath 4X One-Step Multiplex Master Mix (High ROX+)

V5005H DSPath 4X One-Step Multiplex Master Mix (High ROX+)

Price: $300.00~7500.00
V5005H(200 rxns) V5006H(5000 rxns)

Product Introduction

The DSPathTM 4X One-Step Multiplex Master Mix (High ROX+) is used to perform one-step multiplex real-time PCR applications with any gene-specific primer and probe sets in quantitative instruments that require high concentrations of ROX, and is suitable for both RNA and DNA targets. This master mix is formulated with optimized buffer components to accommodate multiplex amplification of up to four RNA or DNA target sequences in a single reaction. The master mix is supplied at a 4X concentration that allows to input more sample into each reaction, increasing sensitivity even in low-volume reactions.

 

We offer 4 formulations of this master mix, which have different buffers to meet amplification requirements of different primers:

Product Name

Cat. No.

Spec.

Quantity  

DSPathTM 4X One-Step Multiplex Master Mix

V5005

V5006

200 rxns

5000 rxns

1 mL × 1

25 mL × 1

DSPathTM 4X One-Step Multiplex Master MixB

V5005B

V5006B

200 rxns

5000 rxns

1 mL × 1

25 mL × 1

DSPathTM 4X One-Step Multiplex Master MixC

V5005C

V5006C

200 rxns

5000 rxns

1 mL × 1

25 mL × 1

DSPathTM 4X One-Step Multiplex Master MixD

V5005D

V5006D

200 rxns

5000 rxns

1 mL × 1

25 mL × 1

Easy to use

Simply add primers, probes, and RNA/DNA templates for rapid reactions.

1703150885483.jpg

Figure 1. One-Step RT-qPCR workflow using DSPathTM 4X One-Step Multiplex Master Mix

 

Selection Guide of One-Step Kit (probe based):

Cat. No.


V5001/V5002

V5005/V5006

V5005L/V5006L

V5007/V5008

V5007L/V5008L

V5009/V5010

V5011

Product Name


One-step Probe RT-qPCR Kit

DSPathTM 4X One-Step Multiplex Master Mix

DSPathTM 4X One-Step Multiplex Master Mix (Lyophilized)

2X One Step Prime RT-qPCR Mix

2X One Step Prime RT-qPCR Mix (Lyophilized)

One-step Probe RT-qPCR Kit V2

One-step Probe RT-qPCR Kit V3

Mixture Format


Enzyme Mix+2X Buffer Mix

4X All-in Mix

4X All-in Lyophilized Powder

2X All-in Mix

2X All-in Lyophilized Powder

Enzyme Mix+2X Buffer Mix

Enzyme Mix+5X Buffer Mix

Mode/Time


S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~80 min

F: ~45 min S: ~80 min

RT TEMP


50~55°C

48~55°C

48~55°C

48~55°C

48~55°C

50~55°C

50~55°C

PCR Enzyme


Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Heat-labile UDG


+

+

+

+

+

+

+

Sensitivity


Very high

Very high

Very high

Very high

Very high

Ultra high

Ultra high

S: standard mode; F: fast mode

V5005, V5009, and V5011 are recommended

Application

Probe gene expression analysis

Probe Low-copy gene detection

Probe microarray validation

Probe gene knockdown validation

Features

Robust one-step, all-in-one master mix system for easy reaction assembly

Detect multiple targets in one reaction

High sensitivity to detect low-copy targets

Tolerance of inhibitors commonly found in clinical samples

Eliminates the risk of cross contamination associated with two-step RT-qPCR protocols

Premixed with high concentration of ROX reference dye


Experimental Cases

1. Comparable to first-line brands

Compare with 6 different brands of similar products to detect samples containing the novel coronavirus at low or high concentrations, #V5005 has excellent detection rate and accuracy.

1703150917213.jpg

Figure 2. detection of low SARS-CoV-2 sample

1703150934963.jpg

Figure 3. detection of high SARS-CoV-2 sample


Stable performance

DSPath 4X One-Step Multiplex Master Mix is very stable, and you can get the benefit from high stability: keep the master mix in the refrigerator for up to 4 weeks and profit from a quick setup without thawing first.

The stability test of DSPath 4X One-Step Multiplex Master Mix/MixB/MixC/MixD was performed at 37°C for 8 days by detecting four targets of SARS-CoV-2. The amplification efficiency of the four products did not change significantly, which means all of them could maintain high stability, and are easy to be transported and stored for a long time.

1703150970030.jpg

Figure 4. amplification curve of stability test of DSPathTM 4X One-Step Multiplex Master Mix/MixB/MixC/MixD

1703150997432.jpg1703151002189.jpg

1703151006478.jpg1703151012157.jpg

Figure 5. Ct value of stability test of DSPathTM 4X One-Step Multiplex Master Mix/MixB/MixC/MixD

Components

Component

V5005H

(200 rxns)

V5006H

(5000 rxns)

DSPath 4X Multiplex Master Mix (High ROX+)

1 m× 1 

25 ml × 1 

Storage Condition

Store at -20°C


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