V5001 One-step Probe RT-qPCR Kit

Products & Applications > 1-step RT-qPCR (probe-based) > V5001 One-step Probe RT-qPCR Kit

V5001 One-step Probe RT-qPCR Kit

Price： $200.00~4000.00

Cat. No./Sp：

V5001(200 rxns) V5002(5000 rxns)

Product details

Product Introduction

The One-step Probe RT-qPCR Kit is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55°C, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Integrating the superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0.1 pg total RNA or <10 copies of RNA template. The kit is available as a convenient Master Mix. 2 × One-step U+ Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity detection systems with fluorescent labeled probes such as TaqMan®. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.

Selection Guide of One-Step Kit (probe based):

Cat. No.	V5001/V5002	V5005/V5006	V5005L/V5006L	V5007/V5008	V5007L/V5008L	V5009/V5010	V5011
Product Name	One-step Probe RT-qPCR Kit	DSPath^TM 4X One-Step Multiplex Master Mix	DSPath^TM 4X One-Step Multiplex Master Mix (Lyophilized)	2X One Step Prime RT-qPCR Mix	2X One Step Prime RT-qPCR Mix (Lyophilized)	One-step Probe RT-qPCR Kit V2	One-step Probe RT-qPCR Kit V3
Mixture Format	Enzyme Mix+2X Buffer Mix	4X All-in Mix	4X All-in Lyophilized Powder	2X All-in Mix	2X All-in Lyophilized Powder	Enzyme Mix+2X Buffer Mix	Enzyme Mix+5X Buffer Mix
Mode/Time	S: ~120 min	F: ~60 min S: ~120 min	F: ~60 min S: ~120 min	F: ~60 min S: ~120 min	F: ~60 min S: ~120 min	F: ~60 min S: ~80 min	F: ~45 min S: ~80 min
RT TEMP	50~55°C	48~55°C	48~55°C	48~55°C	48~55°C	50~55°C	50~55°C
PCR Enzyme	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq
Heat-labile UDG	+	+	+	+	+	+	+
Sensitivity	Very high	Very high	Very high	Very high	Very high	Ultra high	Ultra high

S: standard mode; F: fast mode

V5005, V5009, and V5011 are recommended

Application

• Probe gene expression analysis

• Probe Low-copy gene detection

• Probe microarray validation

• Probe gene knockdown validation

Features

• One-step RT-qPCR

• This kit is suitable for fluorescence quantification by probe method

• This kit is compatible with many real-time systems

• Hot-start technology brings high specificity and reproducible amplification

• dUTP/UDG system, effectively prevent PCR product contamination

Components

Component	V5001 (200 rxns)	V5002 (5000 rxns)
2X One-step U⁺ Mix	760 μl × 4	38.4 ml × 2
One-step U⁺ Enzyme Mix	308 μl	7.7 ml
RNase-free ddH₂O	800 μl × 4	76 ml
50X ROX Reference Dye 1	120 μl	1 ml × 3
50X ROX Reference Dye 2	120 μl	1 ml × 3