Super ECL Kit
(Ultrasensitive Chemiluminescence Detection Kit)
Cat. No./Spec.
D1001/10 ml, D1002/100 ml, D1003/500 ml
Component
Components | D1001 | D1002 | D1003 |
A Solution | 5 ml | 50 ml | 250 ml |
B Solution | 5 ml | 50 ml | 250 ml |
Storage Condition
Store at 4°C, sealed, and protected from light. For short-term storage, it can be kept at room temperature.
Product Description
The Super ECL Kit is used for detecting antibodies labeled with horseradish peroxidase (HRP) in Western Blotting experiments. This product's immunoblotting substrate is an enhanced chemiluminescence (ECL) HRP substrate that enables the detection of low-expressed or high-value proteins in immunoblotting analysis. This ECL substrate is compatible with various membranes, blocking solutions, and a wide range of antibody dilutions.
Product Features
1. Extremely high sensitivity: Picogram-level sensitivity;
2. Strong compatibility: Can detect protein bands on nitrocellulose or PVDF membranes;
3. Long signal duration: Under optimized conditions, the blot bands incubated with the substrate can emit a detectable light signal for 6 to 8 hours;
4. High stability: The working solution remains stable for up to 24 hours;
5. Economical pricing: The formulation is optimized for the detection of antibodies at very low concentrations
- 0.2-1.0 μg/mL primary antibody (or 1 mg/mL dilution range 1:1,000-1:5,000)
- 10-50 ng/mL secondary antibody (or 1 mg/mL dilution range 1:20,000-1:100,000)