T7 RNA Polymerase E1022-A

T7 RNA Polymerase

Cat. No./Spec.: E1022-A/5,000 U 

Concentration: 20 U/µL

Product Description 

Phage T7 RNA polymerase is a DNA-dependent RNA polymerase that has strict specificity for its corresponding double-stranded promoter. It catalyzes the synthesis of RNA in the 5'→3' direction downstream of the promoter, starting from the promoter on single-stranded DNA or double-stranded DNA.

Components

Storage Condition & Shelf Life

Store at -20°C.

Source

Recombinant E. coli strain containing the cloned gene encoding this enzyme.

Unit Definition

A unit is defined as the amount of enzyme required to incorporate 1 nmol of AMP into a polynucleotide substrate within 60 minutes at 37°C.

Features

- Capable of incorporating modified nucleotides (such as aminoallyl, biotin, fluorescein, digoxigenin-labeled nucleotides).

Scope of Application 

The synthesis of unlabeled and labeled RNA can be used for:

• Hybridization, in vitro RNA translation

• As a substrate in aRNA, siRNA, RNase protection assays, and as a template for genomic DNA sequencing

• RNA splicing in studies of RNA secondary structure and RNA-protein interactions.