DNA Polymerase I E1021-A E1021-B

DNA Polymerase I

Cat. No./Spec.: E1021-A/500 U, E1021-B/2,500 U 

Concentration: 10 U/µL

Product Description 

This product is a template-dependent DNA polymerase capable of accelerating the synthesis of DNA in the 5'→3' direction. It also possesses 3'→5' exonuclease (proofreading) activity, 5'→3' exonuclease activity, and RNase H activity.

Components

Storage Condition & Shelf Life

Store at -20°C.

Source

Recombinant E. coli strain containing the cloned gene polA from E. coli.

Unit Definition

A unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of deoxynucleotides into a polynucleotide substrate within 30 minutes at 37°C.

Features

- Incorporation of modified nucleotides (such as biotin, digoxigenin, or fluorescently labeled nucleotides).

- Compatible with a variety of buffer systems, including buffers for restriction enzymes, PCR, and RT reactions.

Scope of Application 

- Used in conjunction with DNase, for DNA labeling through nick translation.

- Used in conjunction with RNase H, for the synthesis of the second strand of cDNA.