T4 β-glucosyltransferase E1020-A

T4 β-glucosyltransferase

Cat. No./Spec.: E1020-A/500 U 

Concentration: 5 U/µL

Product Description 

T4 β-glucosyltransferase (T4 BGT) is capable of transferring the glucosyl group from uridine diphosphate glucose (UDP-glucose) to the 5-hydroxymethylcytosine (5-hmC) residues within double-stranded DNA, resulting in the formation of β-glucosyl-5-hydroxymethylcytosine. This enzyme is formulated for rapid reaction times without compromising the efficiency of the reaction. It can glucosylate 5-hmC on 1 μg of DNA in 15 minutes at 37°C.

Components

Storage Condition & Shelf Life

Store at -20°C.

Unit Definition

A unit is defined as the amount of enzyme required to protect 0.5 μg of fully 5-hydroxymethylated 1095bp PCR fragment from digestion by MunI within 1 hour at 37°C in a 50 μL volume of the recommended reaction buffer.

Features

- Specificity - Selectively transfers glucose to the hydroxymethyl group of 5-hmC.

- Speed - Completes the glucosylation of 1 μg DNA in 15 minutes.

- Convenience - Comes with optimized buffer and UDP-glucose included.

Scope of Application 

- Site-specific detection of 5-hmC.

- Enrichment of DNA containing 5-hmC.

- Labeling of 5-hmC residues using radioactive UDP-glucose donor.