Product Introduction
ARMS qPCR Mix is a 2× qPCR Mix solution developed for the detection of low-frequency variants in tumors, containing highly specific HotStart Taq DNA polymerases, dNTPs, buffers, and other essential components for PCR amplification. The reaction system of the ARMS qPCR Mix has been specially optimized to reduce the formation of mismatched primer amplification products, significantly improve the specificity of PCR amplification, and effectively detect variants as low as 1%. The system contains stabilizers, and repeated freeze-thaw does not affect the amplification performance.
The reaction system of this product can be prepared at room temperature without an ice box. The prepared PCR reaction system can be placed at room temperature for 24 hours and the amplification efficiency remains unchanged.
Application
• SNPs detection
• genotyping
Features
• Convenient: only primers, probes and template DNA are added when preparing qPCR
• High specificity: hotstart Taq DNA Polymerase
• High stability: the performance is not easy to change
• High sensitivity: efficient detection of mutations
Experimental Cases
ARMS PCR Mix has been successfully used for bird sex determination.
Components
Component | P4031 | P4032 |
2X ARMS qPCR Mix | 1 ml | 1 ml × 5 |
Nuclease-free water | 1 ml | 1 ml × 5 |
Storage Condition
Store at -20°C