N9016 Proteinase K Powder

Product Introduction

Molecular Biology Grade，For Research Use Only

Cat No. N9016

Size: 100mg

Specific Activity: ≥30 U/mg protein

Form: Contains proteinase K (≥95%), lyophilized powder

Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. The Proteinase K is classified as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

Source

From yeast cells with cloned gene encoding genetically engineered Engyodontium album (Tritirachium album) endolytic protease.

Molecular Weight

29.3 kDa monomer.

Definition of Activity Unit

One unit of the enzyme liberates Folin-positive amino acids and peptides corresponding to 1 µmol tyrosine in 1 min at 37°C , pH 7.5 using denatured hemoglobin as substrate.

Enzyme activity is assayed in the following mixture: 0.08 M potassium phosphate (pH 7.5), 5 M urea, 4 mM NaCl, 3 mM CaCl2 and 16.7 mg/ml hemoglobin.

Application

• Isolation of genomic DNA from cultured cells and tissues

• Removal of DNases and RNases when isolating DNA and RNA from tissues or cell lines

• Determination of enzyme localization

• Improving cloning efficiency of PCR products

Features

• Recombinant proteinase K

• Active in a wide range of reaction products 

Components

Storage Condition

-20ºC recommended. The shelf life of proteinase K lyophilized powder is three years when stored sealed and dry below 4ºC. Proteinase K lyophilized powder is shipped at room temperature.