GDSlyo One-step Probe RT-qPCR Kit V5013-A V5013-B

Products & Applications > 1-step RT-qPCR (probe-based) > GDSlyo One-step Probe RT-qPCR Kit V5013-A V5013-B

GDSlyo One-step Probe RT-qPCR Kit V5013-A V5013-B

Price： $338.00~5538.00

Cat. No./Sp：

V5013-A (200 rxns) V5013-B (1000 rxns) V5013-C (5000 rxns)

Product details

Product Introduction

The GDSlyo One-step Probe RT-qPCR Kit is a glycerol-free one-step RT-qPCR reagent containing lyophilized excipients for the detection of RNA templates that can be lyophilized with a suitable lyophilization process. The difficulties in the development of lyophilized products include three aspects:1. The removal of liquid reagents affects the lyophilized components, and the stability is poor; 2. It is difficult to freeze-dry and mold, and the yield rate is low; 3. Poor long-term storage stability of lyophilized finished products. Through the optimization of functional proteins, the matching and screening of excipients and lyophilization processes, this product successfully solves the above three problems. Through the screening of enzymes and excipients with high stability, the storage stability of this product is consistent with that of glycerin-containing products; By selecting the appropriate lyophilized excipients and matching the lyophilization process, the yield and stability of the lyophilized products are improved, so that the lyophilized products can be stored at room temperature for more than one year. In addition, this product offers the advantages of conventional glycerol reagents, including good multiplex amplification performance, high specificity, and high sensitivity. The dUTP/UDG anti-contamination system is incorporated into the reagents, which can be used at room temperature to eliminate the influence of amplification product contamination on qPCR results and ensure the accuracy of results.

Cat. No./Spec.

Cat. No.	V5013-A	V5013-B	V5013-C
20-μl reaction Nos.	200 rxns	1,000 rxns	5,000 rxns

Components

Component	V5013-A	V5013-B	V5013-C
5X GDSlyo One-step Buffer ^[1]	800 μl	4 ml	20 ml
GDSlyo One-step Enzyme Mix ^[2]	200 μl	1 ml	5 ml
3X Excipientc ^[3]	1.2 ml	6 ml	30 ml
50X ROX Reference Dye 1 ^[4]	80 μl	400 μl	1 ml × 2
50X ROX Reference Dye 2 ^[4]	80 μl	400 μl	1 ml × 2
RNase-free ddH₂O	1 ml × 3	15 ml	75 ml

[1] Contains dNTP/dUTP mix, Mg²⁺.

[2] Contains Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and Taq DNA Polymerase.

[3] Lyophilized excipients, protect the activity of enzymes during the lyophilization process, maintain the morphology and long-term storage stability of lyophilized products.

[4] Used to correct the fluorescence signal error between holes. 50X ROX Reference Dye 1 is used for ABI 7900HT/ 7300 real-time PCR System and StepOne Plus; ABI 7500, 7500 Fast real-time PCR System, Stratagene Mx3000P using 50X ROX Reference Dye 2; Roche, bio-rad Real Time PCR Systems do not have to use ROX.

Storage

This reagent should be kept at -20°C.

Selection Guide of One-Step Kit (probe based):

Cat. No.	V5001/V5002	V5005/V5006	V5005L/V5006L	V5007/V5008	V5007L/V5008L	V5009/V5010	V5011
Product Name	One-step Probe RT-qPCR Kit	DSPath^TM 4X One-Step Multiplex Master Mix	DSPath^TM 4X One-Step Multiplex Master Mix (Lyophilized)	2X One Step Prime RT-qPCR Mix	2X One Step Prime RT-qPCR Mix (Lyophilized)	One-step Probe RT-qPCR Kit V2	One-step Probe RT-qPCR Kit V3
Mixture Format	Enzyme Mix+2X Buffer Mix	4X All-in Mix	4X All-in Lyophilized Powder	2X All-in Mix	2X All-in Lyophilized Powder	Enzyme Mix+2X Buffer Mix	Enzyme Mix+5X Buffer Mix
Mode/Time	S: ~120 min	F: ~60 min S: ~120 min	F: ~60 min S: ~120 min	F: ~60 min S: ~120 min	F: ~60 min S: ~120 min	F: ~60 min S: ~80 min	F: ~45 min S: ~80 min
RT TEMP	50~55°C	48~55°C	48~55°C	48~55°C	48~55°C	50~55°C	50~55°C
PCR Enzyme	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq
Heat-labile UDG	+	+	+	+	+	+	+
Sensitivity	Very high	Very high	Very high	Very high	Very high	Ultra high	Ultra high