Product Introduction
RT-PCR Mix for qPCR is a premixed reverse transcription reaction (RT-PCR) kit, easy to operate, can quickly and efficiently complete the reverse transcription reaction. RT-PCR Enzyme Mix contains highly efficient reverse transcriptase and heat labile DNase, which can complete genomic DNA clearance and RT-PCR of RNA in one step. 5X RT-PCR Buffer contains other components required for RT-PCR, without the need for multiple addition of components step by step, thus reducing the risk of sample contamination. At the same time, the kit is equipped with Control Mix for a negative control reaction to test for genomic DNA residue in the RNA template. The reverse transcription products of this kit have good compatibility for real-time PCR (qPCR) reaction by dye method and probe method, and can be used for accurate gene expression analysis.
Application
First strand cDNA synthesis.
Features
Buffer mix + enzyme mix, easy to use
Components
Component | R1031 |
RT-PCR Enzyme Mix | 100 μl |
5X RT-PCR Buffer | 400 μl |
Control Mix | 20 μl |
RNase-free ddH2O | 1 ml × 2 |
Storage Condition
Store at -20°C