R1011 RT - PCR Kit

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R1011 RT - PCR Kit

Price： $60.00~285.00

Cat. No./Sp：

R1011(20 rxns) R1012(100 rxns)

Product details

Product Introduction

RT-PCR Kit is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. The RT-PCR Kit for RT-PCR delivers increased cDNA yields, high sensitivity, and full-length transcripts in a convenient format. You get all of the components you need for successful first-strand cDNA synthesis, saving your time and ensuring your success with every experiment. The kit Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42-55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases.

Application

First strand cDNA synthesis for RT-PCR

Construction of cDNA libraries

One-step RT-PCR

Primer extension

Features

Each component individually packaged, flexible dosage

Components

Component	R1011	R1012
M-MLV (200U/μl)	20 μl	100 μl
RNasin (40U/μl)	12 μl	60 μl
Oligo(dT)₁₅(50μM)	20 μl	100 μl
Random hexamer primer (50μM)	20 μl	100 μl
5× First-strand Buffer	80 μl	400 μl
RNase-free ddH₂O	1 ml	1 ml × 5
dNTPs(10mM each)	50 μl	250 μl