Super 4X One-Step Multiplex Master Mix V5012-A V5012-B

Product Introduction

The Super 4X One-Step Multiplex Master Mix is used to perform one-step multiplex real-time PCR applications with any gene-specific primer and probe sets, and is suitable for both RNA and DNA targets. Dual hot-start reverse transcriptase and DNA polymerase can complete more than 10-plex highly sensitive and specific quantitative PCR in a single system. The master mix is supplied at a 4X concentration that allows to input more sample into each reaction, increasing sensitivity even in low-volume reactions.

Selection Guide of One-Step Kit (probe based):

S: standard mode; F: fast mode

V5012, V5009, and V5011 are recommended

Application

• Probe gene expression analysis

• Probe Low-copy gene detection

• Probe microarray validation

• Probe gene knockdown validation

Features

• support 10-plex amplification with high specificity

• Reverse transcription at 55 °C to accommodate complex RNA templates

• Contains heat-labile UDG to reduce false-positive contamination

• High sensitivity for detection of low-abundance genes

• High stability

• 4X formation, high concentration

• Saves time by supporting fast program

Components

Storage Condition

This reagent should be kept at -15~-25°C.