Product Introduction
Power Green One-step RT-qPCR Kit is a one-step reverse transcriptation-fluorescence quantitative PCR kit. This product contains reverse transcriptase, Taq DNA polymerase, dNTPs, SYBR Green I dye and other reagents required for reverse transcription and qPCR reaction. By adding only RNA (such as viral RNA) and primers, reverse transcription and dye qPCR can be performed in the same reaction tube. This product reduces the steps of experimental operation, not only improves the efficiency of detection, but also reduces the risk of contamination. Kits are available in convenient Master Mix form. Power Green 1-step Enzyme Mix contains heat-resistant reverse transcriptase and antibody modified hotstart DNA polymerase, which can maintain stable reverse transcriptase activity at 55°C to ensure high reverse transcriptase efficiency. hotstart Taq combined with optimized buffering system ensures high sensitivity and specificity of Power Green One-step RT-qPCR Kit. 2X Power Green 1-step Reaction Mix contains an optimized buffer system and SYBR Green I dye for SYBR/FAM channel detection.
Application
SYBR green I dye-based One-step RT-qPCR
Features
All-in-one master mix, easy to use and saving time
heat-resistant reverse transcriptase and antibody modified hotstart DNA polymerase
High efficiency of reverse transcription and qPCR
high sensitivity and specificity
Components
Component | V6001-A (200 rxns) | V6001-B (200 rxns) |
2X Power Green 1-step Reaction Mix | 1 ml × 2 | 25 ml × 2 |
Power Green 1-step Enzyme Mix | 200 μl | 1 ml × 5 |
Storage Condition
Store at -20°C away from light