P1061 Long Taq DNA Polymerase

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P1061 Long Taq DNA Polymerase

Price： $19.00~73.00

Cat. No./Sp：

P1061(250 U) P1062（250 U (dNTPs+)） P1063(1,000 U) P1064(1,000 U (dNTPs+))

Product details

Product Introduction

Long Taq DNA Polymerase, a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying long DNA fragments. This specially formulated Long Taq DNA Polymerase was shown to amplify long DNA templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich template.

Long Taq DNA Polymerase is suitable as a replacement for ordinary Taq Polymerase in most applications. The elongation rate is 3kb/min. The products of Long Taq DNA Polymerase is a mix of 3´-dA overhangs and blunt-ends products, which can be used in TA clone.

Application

• PCR amplification of complex template DNA

• PCR amplification of long DNA sequences

• DNA sequencing

• PCR for cloning

Features

• High fidelity: 3X fidelity of Taq DNA Polymerase.

• Longer fragment: amplify long templates as long as 20 kb.

• Amplification of complex template (GC rich or repetitive sequence).

• Generates 3'-dA and blunt-end PCR products.

Components

Component	P1061 (250U)	P1062 (250U)	P1063 (1000U)	P1064 (1000U)
Long Taq DNA Polymerase (5U/μl)	50 μl	50 μl	200 μl	200 μl
10× Long PCR Buffer Ⅰ (Mg2+ Plus)*	1.25 ml	1.25 ml	1.25 ml × 2	1.25 ml × 2
10× Long PCR Buffer Ⅱ (Mg2+ Plus) *	1.25 ml	1.25 ml	1.25 ml × 2	1.25 ml × 2
dNTPs (2.5mM each)	-	1 ml	-	1 ml × 2
PCR Enhancer	500 μl	500 μl	500 μl × 2	500 μl × 2
6× Loading Buffer	1 ml	1 ml	1 ml	1 ml