Product Introduction
2× FS Mix is a premixed, ready-to-use solution containing hotstart FS Taq DNA Polymerase with antibody modification, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. This premixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. It also contributes to higher sensitivity by adding enhancer.
Application
Colony PCR; Rapid PCR; Genotyping
Features
• Convenient: only primers and template DNA are added when preparing PCR system
• Fast elongation: elongation rate can reach to 3kb/min, 3 times rate of Taq DNA Polymerase
• Thermostable: half-life over 40 min at 95°C incubation
• Generates 3'-dA overhangs PCR products
Experimental Cases
After the upgrade, FS Mix significantly improves PCR amplification efficiency.
Components
Component | P2071 | P2072 |
2× FS Mix* | 1 ml | 1 ml × 5 |
Nuclease-free water | 1 ml | 1 ml × 5 |
* Available in two versions: bromophenol blue plus and bromophenol blue free (suggested).
Storage Condition
Store at -20°C