Product Introduction
Super HIFI PCR Master Mix is a PCR premix containing hotstart proofreading DNA polymerase and contains the various components (except primers and templates) required for PCR reactions. The chemically modified ultra-high fidelity DNA polymerase in this Master Mix is extremely specific, with 100-fold fidelity of Taq. It can be used for molecular cloning, first-generation sequencing, site-directed mutagenesis, NGS library construction and other PCR requiring high accuracy. This amplification reagent has undergone strict quality control and functional verification to ensure the stability and reproducibility of the reagent to the greatest extent. This product is suitable for multiple amplification and amplicon library construction with initial DNA template of 1-100 ng, and is compatible with DNA templates from different sample types: cell or tissue DNA, formalin-fixed paraffin embedded (FFPE) sample DNA, cell-free DNA(cfDNA), etc.
Application
• High-specificity amplification & multiplex PCR
• High fidelity PCR
• NGS library preparation
Features
• 100X ultra-high fidelity of Taq
• hotstart with chemical modification
• Suitable for NGS multiplex PCR
Components
Component | P2111 (100 rxns) | P2112 (1000 rxns) | P2113 (5000 rxns) |
2X Super HIFI PCR Master Mix | 1 ml × 1 | 1 ml × 10 | 10 ml × 5 |
GC Enhancer | 0.25 ml × 1 | 1 ml × 2 | 10 ml × 1 |
Storage Condition
Store at -20°C