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P1011 Taq DNA Polymerase

Products & Applications > Molecular Enzyme > P1011 Taq DNA Polymerase

P1011 Taq DNA Polymerase

Price: $6.70~176.00
P1011(500 U) P1012(500 U (dNTPs+)) P1013(1,000 U) P1014(1,000 U (dNTPs+)) P1015(18,000 U)

Product Introduction

Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.

Application

• PCR amplification of DNA fragments as long as 5 kb

• DNA labeling

• DNA sequencing

• PCR for cloning

Features

Classical and conventional Taq DNA Polymerase, 2kb/min, generates 3'-dA overhangs

Components

Component

P1011

(500U)

P1012

(500U)

P1013

(1000U)

P1014

(1000U)

P1015

(18000U)

Taq DNA Polymerase (5U/μl)

100 μl

100 μl

200 μl

200 μl

100 μl × 36

10× PCR Buffer (Mg2+ Plus)* 

1.25 ml

1.25 ml

1.25 ml × 2

1.25 ml × 2

1.25 ml × 36

dNTPs (2.5mM each)

-

1 ml

-

1 ml × 2

-

6× Loading Buffer

1 ml

1 ml

1 ml

1 ml

-

* Mg2+ Free version is also available.

 

Storage Condition

Store at -20°C


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