Product Introduction
Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.
Application
• PCR amplification of DNA fragments as long as 5 kb
• DNA labeling
• DNA sequencing
• PCR for cloning
Features
Classical and conventional Taq DNA Polymerase, 2kb/min, generates 3'-dA overhangs
Components
Component | P1011 (500U) | P1012 (500U) | P1013 (1000U) | P1014 (1000U) | P1015 (18000U) |
Taq DNA Polymerase (5U/μl) | 100 μl | 100 μl | 200 μl | 200 μl | 100 μl × 36 |
10× PCR Buffer (Mg2+ Plus)* | 1.25 ml | 1.25 ml | 1.25 ml × 2 | 1.25 ml × 2 | 1.25 ml × 36 |
dNTPs (2.5mM each) | - | 1 ml | - | 1 ml × 2 | - |
6× Loading Buffer | 1 ml | 1 ml | 1 ml | 1 ml | - |
* Mg2+ Free version is also available.
Storage Condition
Store at -20°C