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P1061 Long Taq DNA Polymerase

Products & Applications > PCR > P1061 Long Taq DNA Polymerase

P1061 Long Taq DNA Polymerase

Price: $19.00~73.00
P1061(250 U) P1062(250 U (dNTPs+)) P1063(1,000 U) P1064(1,000 U (dNTPs+))

Product Introduction

Long Taq DNA Polymerase, a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying long DNA fragments. This specially formulated Long Taq DNA Polymerase was shown to amplify long DNA templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich template.

Long Taq DNA Polymerase is suitable as a replacement for ordinary Taq Polymerase in most applications. The elongation rate is 3kb/min. The products of Long Taq DNA Polymerase is a mix of 3´-dA overhangs and blunt-ends products, which can be used in TA clone.

Application

• PCR amplification of complex template DNA

• PCR amplification of long DNA sequences

• DNA sequencing

• PCR for cloning

Features

• High fidelity: 3X fidelity of Taq DNA Polymerase.

• Longer fragment: amplify long templates as long as 20 kb.

• Amplification of complex template (GC rich or repetitive sequence).

• Generates 3'-dA and blunt-end PCR products.

Components

Component

P1061

(250U)

P1062

(250U)

P1063

(1000U)

P1064

(1000U)

Long Taq DNA Polymerase (5U/μl)

50 μl

50 μl

200 μl

200 μl

10× Long PCR Buffer Ⅰ (Mg2+ Plus)* 

1.25 ml

1.25 ml

1.25 ml × 2

1.25 ml × 2

10× Long PCR Buffer Ⅱ (Mg2+ Plus) *

1.25 ml

1.25 ml

1.25 ml × 2

1.25 ml × 2

dNTPs (2.5mM each)

-

1 ml

-

1 ml × 2

PCR Enhancer

500 μl

500 μl

500 μl × 2

500 μl × 2

6× Loading Buffer

1 ml

1 ml

1 ml

1 ml

* Mg2+ Free version is also available. 

Storage Condition

Store at -20°C


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