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Super 4X One-Step Multiplex Master Mix V5012-A V5012-B

Products & Applications > Molecular Diagnostic Raw Materials > Super 4X One-Step Multiplex Master Mix V5012-A V5012-B

Super 4X One-Step Multiplex Master Mix V5012-A V5012-B

Price: $475.00~9350.00
V5012-A(200 rxns) V5012-B(5000 rxns)

Product Introduction

The Super 4X One-Step Multiplex Master Mix is used to perform one-step multiplex real-time PCR applications with any gene-specific primer and probe sets, and is suitable for both RNA and DNA targets. Dual hot-start reverse transcriptase and DNA polymerase can complete more than 10-plex highly sensitive and specific quantitative PCR in a single system. The master mix is supplied at a 4X concentration that allows to input more sample into each reaction, increasing sensitivity even in low-volume reactions.

 

Selection Guide of One-Step Kit (probe based):

Cat. No.


V5001/V5002

V5005/V5006

V5005L/V5006L

V5007/V5008

V5007L/V5008L

V5009/V5010

V5011

V5012

Product Name


One-step Probe RT-qPCR Kit

DSPathTM 4X One-Step Multiplex Master Mix

DSPathTM 4X One-Step Multiplex Master Mix (Lyophilized)

2X One Step Prime RT-qPCR Mix

2X One Step Prime RT-qPCR Mix (Lyophilized)

One-step Probe RT-qPCR Kit V2

One-step Probe RT-qPCR Kit V3

Super 4X One-Step Multiplex Master Mix

Mixture Format


Enzyme Mix+2X Buffer Mix

4X All-in Mix

4X All-in Lyophilized Powder

2X All-in Mix

2X All-in Lyophilized Powder

Enzyme Mix+2X Buffer Mix

Enzyme Mix+5X Buffer Mix

4X All-in Mix

Mode/Time


S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~80 min

F: ~45 min S: ~80 min

F: ~70 min S: ~130 min

RT TEMP


50~55°C

48~55°C

48~55°C

48~55°C

48~55°C

50~55°C

50~55°C

48~55°C

PCR Enzyme


Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Heat-labile UDG


+

+

+

+

+

+

+

+

Sensitivity


Very high

Very high

Very high

Very high

Very high

Ultra high

Ultra high

Ultra high

S: standard mode; F: fast mode

V5012, V5009, and V5011 are recommended

 

Application

Probe gene expression analysis

Probe Low-copy gene detection

Probe microarray validation

Probe gene knockdown validation

 

Features

support 10-plex amplification with high specificity

Reverse transcription at 55 °C to accommodate complex RNA templates

Contains heat-labile UDG to reduce false-positive contamination

High sensitivity for detection of low-abundance genes

High stability

4X formation, high concentration

Saves time by supporting fast program

 

Components

Component

V5012-A

(200rxns) rxns)rxns)

V5012-B

(5000 rxns)

Super 4X One-Step Multiplex Master Mix

1 ml × 1

25 ml × 1 

 

Storage Condition

This reagent should be kept at -15~-25°C.




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