K001 Fast DNA Library Prep Kit

Product Introduction

Aiming at Illumina high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines end repair and A-Tailing into one step, and reagents are highly premixed, greatly shortening the time of library construction and reducing the error caused by tedious steps. The end preparation, adapter ligation, amplification and purification of the fragmented double-stranded DNA can be performed within about 2 hours. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.

Application

• NGS library preparation for Illumina platform

Features

1 Wide sample compatibility

2 High efficiency in library construction

3 Compatible with PCR-free workflow

4 End Repair & A-Tailing Enzyme Mix and Buffer are provided as one tube

Experimental Cases

1. High conversion rate of library.

Figure 1 Library conversion rate when DNA input was 60ng.

2. Whether before or after amplification, compared with other manufacturers, the amplification is very superior.

Figure 2 The concentration of unamplified library obtained when the DNA sample was 100ng and 1ng, and the end repair and adapter ligation were performed with the library construction kits of different manufacturers.

Figure 3 The total amount of library obtained after amplified with the same cycles when the DNA sample was 100ng, 60ng, and 20ng, and the end repair and adapter ligation were performed with the library construction kits of different manufacturers.

Components

Storage Condition

All reagents should be stored at -20°C.