Product Introduction
GDSPure DNA Selection Magbeads use high-performance super-paramagnetic beads and excellent buffer ratio to accurately purify and select DNA fragments from 150 bp to 1000 bp or even larger. The excess nucleotides, salts, enzymes and other impurities introduced in the operation of DNA library construction will be removed after simple washing process, so as to obtain purified fragments, which can be directly used in downstream applications such as sequencing, hybridization, PCR and enzyme digestion. GDSPure DNA Selection Magbeads can be used by manual and automatic formats.
Application
Size selection and cleanup for Next-generation sequencing (NGS), Sanger sequencing, qPCR, ddPCR and microarrays, etc.
Features
• High recovery of amplicons ≥ 150bp
•Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
• Predictable and consistent size selection
• Compatible with manual and automated processing
Experimental Cases
Size Selection Workflow
Selection of DNA fragments longer than a certain size (single round of selection/purification)
GDS: GDSPure DNA Selection Magbeads,BM: A foreign brand similar reagent
M:GSDBio 50 bp Ladder
0.8X, 1X, 1.2X, 1.6X: Respectively use 0.8X, 1X, 1.2X, 1.6X GDSPure magnetic bead to recover nucleic acid fragments of various sizes
Selection of DNA fragments in a certain size range (two rounds of selection)
GDS: GDSPure DNA Selection Magbeads, BM: A foreign similar reagent
M: GSDBio 50 bp Ladder, 100bp Ladder
S1,S2: Recovery range near 250 bp; S3,S4: Recovery range near 200 bp; S5,S6:Recovery range near 300 bp; S7,S8: Recovery range near 400 bp; S9,S10: Recovery range near 500 bp; S11,S12: Recovery range near 600 bp
Components
Component | NC1011 | NC1012 | NC1013 |
GDSPure DNA Selection Magbeads | 5 mL | 60 mL | 450 mL |
Storage Condition
This reagent should be kept at 2-8°C. Do not freeze.