NGS Multiplex PCR Master MixIII NM3001/NM3002/NM3003

Product Introduction

NGS Multiplex PCR Master MixIII is a PCR Master Mix designed for unbiased targeted amplification in next-generation sequencing (NGS). By optimizing enzymes and buffers, this product significantly improves the success rate and consistency of amplification efficiency for GC/AT-rich amplicons. Primers with different GC contents can use a uniform annealing temperature, enabling unbiased PCR amplification, up to 10,000X multiplex PCR, and can tolerate PCR inhibitors such as SDS, guanidine salts, and heparin. The product contains green, blue, and yellow dye reagents, allowing direct electrophoresis after PCR reaction without the need for additional dye addition. It has a fidelity more than 100 times higher than Taq and can be used for targeted NGS sequencing of viral, microbial, tumor/heritable DNA.

Application

This product is suitable for PCR amplification of NGS libraries.

Features

Stale amplification efficiency.

100X Taq fidelity.

Supports 10,000x multiplex PCR.

Tolerates various sample inhibitors.

Components

Storage Condition

Store unopened at –15°C to –25°C until the expiration date on the label. After opening, the master mix may be stored at –15°C to –25°C until the expiration date on the label, or at 4°C for up to 30 days.