Universal DNA Extraction Kit N1211/N1212

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Universal DNA Extraction Kit N1211/N1212

Price： $25.80~92.30

Cat. No.：

N1211/24preps N1212/100preps

Product details

Universal DNA Extraction Kit

‍(Silicone Membrane Centrifugal Column Method)

Cat. No./Spec.: N1211/24 preps; N1212/100 preps

Kit Contents

Component	N1211	N1212
Buffer ATL	10 ml	30 ml
Buffer AL	10 ml	30 ml
Buffer SW1	20 ml	60 ml
Buffer SW2	30 ml	110 ml
Proteinase K	0.6 ml	2.5 ml
Elution Buffer	10 ml	20 ml
DNA Spin Column	24 pcs	100 pcs
Collection Tube	24 pcs	100 pcs

Description

This product utilizes column purification technology, suitable for extracting high-purity DNA from animal tissues, processed animal-derived samples, cultured cells, exfoliated cells, blood, saliva, swabs, bloodstains, bacteria, and other samples. The purification principle involves lysing cells with cell lysis solution to release genomic DNA, which is then reversibly adsorbed by the silicone membrane column. After digestion with proteinase K and washing with wash solution to remove proteins, lipids, and polysaccharides, the genomic DNA is eluted with pure water to obtain the genomic DNA. The extraction process does not require the use of toxic phenol-chloroform extraction or time-consuming alcohol precipitation. The digestion process after extraction only takes 20 minutes. The obtained DNA can be directly used for PCR, quantitative PCR, Southern Blot, viral DNA detection, and second-generation sequencing experiments.

Storage

This product can be stored at room temperature (15~25 ̊C) for 18 months. Buffer ATL may precipitate at low temperatures, which can be dissolved by incubating at 55ºC and mixing thoroughly. The product uses PET reagent bottles, which should not be subjected to high-temperature, high-pressure sterilization or direct incubation above 55ºC.