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V5005H DSPath 4X One-Step Multiplex Master Mix (High ROX+)

Products & Applications > Molecular Research Reagents > V5005H DSPath 4X One-Step Multiplex Master Mix (High ROX+)

V5005H DSPath 4X One-Step Multiplex Master Mix (High ROX+)

Price： $300.00~7500.00

Cat No/Spec：

V5005H(200 rxns) V5006H(5000 rxns)

Product details

Product Introduction

The DSPath^TM 4X One-Step Multiplex Master Mix (High ROX+) is used to perform one-step multiplex real-time PCR applications with any gene-specific primer and probe sets in quantitative instruments that require high concentrations of ROX, and is suitable for both RNA and DNA targets. This master mix is formulated with optimized buffer components to accommodate multiplex amplification of up to four RNA or DNA target sequences in a single reaction. The master mix is supplied at a 4X concentration that allows to input more sample into each reaction, increasing sensitivity even in low-volume reactions.

We offer 4 formulations of this master mix, which have different buffers to meet amplification requirements of different primers:

Product Name

Cat. No.

Spec.

Quantity

DSPath^TM 4X One-Step Multiplex Master Mix

V5005

V5006

200 rxns

5000 rxns

1 mL × 1

25 mL × 1

DSPath^TM 4X One-Step Multiplex Master MixB

V5005B

V5006B

200 rxns

5000 rxns

1 mL × 1

25 mL × 1

DSPath^TM 4X One-Step Multiplex Master MixC

V5005C

V5006C

200 rxns

5000 rxns

1 mL × 1

25 mL × 1

DSPath^TM 4X One-Step Multiplex Master MixD

V5005D

V5006D

200 rxns

5000 rxns

1 mL × 1

25 mL × 1

Easy to use

Simply add primers, probes, and RNA/DNA templates for rapid reactions.

Figure 1. One-Step RT-qPCR workflow using DSPath^TM 4X One-Step Multiplex Master Mix

Selection Guide of One-Step Kit (probe based):

Cat. No.	V5001/V5002	V5005/V5006	V5005L/V5006L	V5007/V5008	V5007L/V5008L	V5009/V5010	V5011
Product Name	One-step Probe RT-qPCR Kit	DSPath^TM 4X One-Step Multiplex Master Mix	DSPath^TM 4X One-Step Multiplex Master Mix (Lyophilized)	2X One Step Prime RT-qPCR Mix	2X One Step Prime RT-qPCR Mix (Lyophilized)	One-step Probe RT-qPCR Kit V2	One-step Probe RT-qPCR Kit V3
Mixture Format	Enzyme Mix+2X Buffer Mix	4X All-in Mix	4X All-in Lyophilized Powder	2X All-in Mix	2X All-in Lyophilized Powder	Enzyme Mix+2X Buffer Mix	Enzyme Mix+5X Buffer Mix
Mode/Time	S: ~120 min	F: ~60 min S: ~120 min	F: ~60 min S: ~120 min	F: ~60 min S: ~120 min	F: ~60 min S: ~120 min	F: ~60 min S: ~80 min	F: ~45 min S: ~80 min
RT TEMP	50~55°C	48~55°C	48~55°C	48~55°C	48~55°C	50~55°C	50~55°C
PCR Enzyme	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq
Heat-labile UDG	+	+	+	+	+	+	+
Sensitivity	Very high	Very high	Very high	Very high	Very high	Ultra high	Ultra high

S: standard mode; F: fast mode

V5005, V5009, and V5011 are recommended

Application

• Probe gene expression analysis

• Probe Low-copy gene detection

• Probe microarray validation

• Probe gene knockdown validation

Features

• Robust one-step, all-in-one master mix system for easy reaction assembly

• Detect multiple targets in one reaction

• High sensitivity to detect low-copy targets

• Tolerance of inhibitors commonly found in clinical samples

• Eliminates the risk of cross contamination associated with two-step RT-qPCR protocols

• Premixed with high concentration of ROX reference dye

Experimental Cases

1. Comparable to first-line brands

Compare with 6 different brands of similar products to detect samples containing the novel coronavirus at low or high concentrations, #V5005 has excellent detection rate and accuracy.

Figure 2. detection of low SARS-CoV-2 sample

Figure 3. detection of high SARS-CoV-2 sample

Stable performance

DSPath 4X One-Step Multiplex Master Mix is very stable, and you can get the benefit from high stability: keep the master mix in the refrigerator for up to 4 weeks and profit from a quick setup without thawing first.

The stability test of DSPath 4X One-Step Multiplex Master Mix/MixB/MixC/MixD was performed at 37°C for 8 days by detecting four targets of SARS-CoV-2. The amplification efficiency of the four products did not change significantly, which means all of them could maintain high stability, and are easy to be transported and stored for a long time.