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GDSlyo One-step Probe RT-qPCR Kit V5013-A V5013-B

Products & Applications > Molecular Research Reagents > GDSlyo One-step Probe RT-qPCR Kit V5013-A V5013-B

GDSlyo One-step Probe RT-qPCR Kit V5013-A V5013-B

Price: $338.00~5538.00
V5013-A (200 rxns) V5013-B (1000 rxns) V5013-C (5000 rxns)

Product Introduction

The GDSlyo One-step Probe RT-qPCR Kit is a glycerol-free one-step RT-qPCR reagent containing lyophilized excipients for the detection of RNA templates that can be lyophilized with a suitable lyophilization process. The difficulties in the development of lyophilized products include three aspects:1. The removal of liquid reagents affects the lyophilized components, and the stability is poor; 2. It is difficult to freeze-dry and mold, and the yield rate is low; 3. Poor long-term storage stability of lyophilized finished products. Through the optimization of functional proteins, the matching and screening of excipients and lyophilization processes, this product successfully solves the above three problems. Through the screening of enzymes and excipients with high stability, the storage stability of this product is consistent with that of glycerin-containing products; By selecting the appropriate lyophilized excipients and matching the lyophilization process, the yield and stability of the lyophilized products are improved, so that the lyophilized products can be stored at room temperature for more than one year. In addition, this product offers the advantages of conventional glycerol reagents, including good multiplex amplification performance, high specificity, and high sensitivity. The dUTP/UDG anti-contamination system is incorporated into the reagents, which can be used at room temperature to eliminate the influence of amplification product contamination on qPCR results and ensure the accuracy of results.


Cat. No./Spec.

Cat. No.

V5013-A

V5013-B

V5013-C

20-μl reaction Nos.

200 rxns

1,000 rxns

5,000 rxns

 

Components

Component

V5013-A 

V5013-B 

V5013-C

5X GDSlyo One-step Buffer [1]

800 μl

4 ml

20 ml

GDSlyo One-step Enzyme Mix [2]

200 μl

1 ml 

5 ml

3X Excipientc [3]

1.2 ml

6 ml

30 ml

50X ROX Reference Dye 1 [4]

80 μl

400 μl

1 ml × 2

50X ROX Reference Dye 2 [4]

80 μl

400 μl

1 ml × 2

RNase-free ddH2O

1 ml × 3 

15 ml 

75 ml

[1] Contains dNTP/dUTP mix, Mg2+.

[2] Contains Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and Taq DNA Polymerase.

[3] Lyophilized excipients, protect the activity of enzymes during the lyophilization process, maintain the morphology and long-term storage stability of lyophilized products.

[4] Used to correct the fluorescence signal error between holes. 50X ROX Reference Dye 1 is used for ABI 7900HT/ 7300 real-time PCR System and StepOne Plus; ABI 7500, 7500 Fast real-time PCR System, Stratagene Mx3000P using 50X ROX Reference Dye 2; Roche, bio-rad Real Time PCR Systems do not have to use ROX.

 

Storage

This reagent should be kept at -20°C.



Selection Guide of One-Step Kit (probe based):

Cat. No.


V5001/V5002

V5005/V5006

V5005L/V5006L

V5007/V5008

V5007L/V5008L

V5009/V5010

V5011

Product Name


One-step Probe RT-qPCR Kit

DSPathTM 4X One-Step Multiplex Master Mix

DSPathTM 4X One-Step Multiplex Master Mix (Lyophilized)

2X One Step Prime RT-qPCR Mix

2X One Step Prime RT-qPCR Mix (Lyophilized)

One-step Probe RT-qPCR Kit V2

One-step Probe RT-qPCR Kit V3

Mixture Format


Enzyme Mix+2X Buffer Mix

4X All-in Mix

4X All-in Lyophilized Powder

2X All-in Mix

2X All-in Lyophilized Powder

Enzyme Mix+2X Buffer Mix

Enzyme Mix+5X Buffer Mix

Mode/Time


S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~80 min

F: ~45 min S: ~80 min

RT TEMP


50~55°C

48~55°C

48~55°C

48~55°C

48~55°C

50~55°C

50~55°C

PCR Enzyme


Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Heat-labile UDG


+

+

+

+

+

+

+

Sensitivity


Very high

Very high

Very high

Very high

Very high

Ultra high

Ultra high

S: standard mode; F: fast mode

V5005, V5009, and V5011 are recommended

 

Application

Probe gene expression analysis

Probe Low-copy gene detection

Probe microarray validation

Probe gene knockdown validation

Features

One-step RT-qPCR

This kit is suitable for fluorescence quantification by probe method

This kit is compatible with many real-time systems

Hot-start technology brings high specificity and reproducible amplification

dUTP/UDG system, effectively prevent PCR product contamination


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