P2701 Multiplex Probe qPCR Mix with UDG

Products & Applications > Molecular Research Reagents > P2701 Multiplex Probe qPCR Mix with UDG

P2701 Multiplex Probe qPCR Mix with UDG

Price： $49.00~3690.00

Cat. No./Sp：

P2701(1 mL) P2702(1 mL×5) P2703(50 mL) P2704(100 mL)

Product details

Multiplex Probe qPCR Mix Plus U

For research use only

Cat. No./Spec.

Cat. No.	P2701	P2702	P2703	P2704
25-μl rxn Nos	80 rxns	400 rxns	4000 rxns	8000 rxns

Components

Component	P2701	P2702	P2703	P2704
2× Multiplex Probe qPCR Mix Plus U	1 ml	1 ml × 5	50 ml	100 ml
Nuclease-free Water	1 ml	1 ml × 5	-	-

Storage

This reagent can be stored for 2 months at 4°C. For longer storage, it should be kept at -20°C.

Description

Multiplex Probe qPCR Mix Plus U is a 2× concentrated premix for real-time quantitative PCR with probe method. In use, just add the DNA template, primer and probe to react. This product contains antibody technology modified Hotstart Taq DNA polymerase. Combined with GDSBio's special real-time PCR Buffer, it can not only effectively inhibit primers dimer and other non-specific amplification, but also improve the amplification efficiency, allowing multi-probe qPCR reaction. This reagent introduced dUTP/UDG anti-contamination system, which can remove PCR products containing dUTP before PCR reaction, effectively avoid the influence of cross contamination of amplification products on quantification. This product is convenient for use with TaqMan, primers to achieve a fully premixed Master Mix molecular diagnostic kit, and is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc. This product can be used with TaqMan and other fluorescent probes, and is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-rad, etc.

The reaction system of this product can be prepared at room temperature without an ice box. The prepared PCR reaction system can be placed at room temperature for 24 hours and the amplification efficiency remains unchanged.

Application

• Probe gene expression analysis

• Multiple Low-copy gene detection

• Probe microarray validation

• Probe gene knockdown validation

Features

• This kit is suitable for Multiplex fluorescence quantification by probe method

• This kit is compatible with many real-time systems

• Hot-start technology brings high specificity and reproducible amplification

• dUTP/UDG system, effectively prevent PCR product contamination

Experimental Cases

Using human genome DNA as template, it has good multiplex amplification performance.