Product Introduction
Super SYBR® Green qPCR Mix is a 2X concentrated real-time PCR master mix that requires only the addition of template and primers to perform the reaction. The use of an innovative hotstart mechanism can reduce the interference of primer-dimers and other secondary products on the reaction, which can significantly improve the specificity and amplification efficiency of quantitative PCR, and obtain a wider quantitative amplification region. The new enhancer minimizes fluctuations in PCR efficiency for various target fragments, and repeated freeze-thaw cycles have minimal impact on amplification performance. This product comes with a special ROX Reference Dye, which is suitable for all qPCR instruments, eliminating the need to adjust the concentration of ROX on different instruments.
Application
• Gene expression analysis
• Low-copy gene detection
• Microarray validation
• Gene knockdown validation
Features
• Compatible with common Real-time systems without the need to adjust ROX reference dye
• Hot-start technology brings high specificity and reproducible amplification
Components
Component | P2131 | P2132 | P2133 |
2X Super SYBR Green qPCR Mix | 1 ml | 1 ml × 5 | 1 ml × 10 |
Nuclease-free Water | 1 ml | 1 ml × 5 | - |
Storage Condition
This reagent can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light.