RP1001B 2X One Step RT-PCR Mix

Product Introduction

The 2X One Step RT-PCR Mix is a one-step kit for reverse transcription and endpoint PCR. It amplifies RNA target sequences with gene-specific primers (GSP). Optimized buffer components enable reverse transcription and PCR reactions to be performed within one system. This product is composed of a two-fold concentration of reaction buffer and an enzyme mixture, which is more conducive to the stable preservation of enzyme reagents than a one-tube all-in mix. This product is simple and convenient to use, only GSP and template RNA need to be added to use, and no additional capping and pipetting operations are required, which not only saves time, but also effectively reduces the risk of contamination. This product contains heat labile DNase, which can remove genomic DNA at the same time when reverse transcription. Heat labile DNase is susceptible to high temperature inactivation and is conducive to stable preservation of cDNA or amplification reactions.

Application

1-STEP reverse transcription and endpoint PCR

Features

Enzyme mix + reaction mix, the amount of enzyme can be flexibly adjusted

Heat labile DNase, remove genomic DNA and be inactivated thoroughly at high temperature of PCR

High efficiency of reverse transcription and PCR

Components

Storage Condition

Store at -20°C