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Super 4X One-Step Multiplex Master Mix V5012-A V5012-B
Super 4X One-Step Multiplex Master Mix V5012-A V5012-B
Product Introduction
The Super 4X One-Step Multiplex Master Mix is used to perform one-step multiplex real-time PCR applications with any gene-specific primer and probe sets, and is suitable for both RNA and DNA targets. Dual hot-start reverse transcriptase and DNA polymerase can complete more than 10-plex highly sensitive and specific quantitative PCR in a single system. The master mix is supplied at a 4X concentration that allows to input more sample into each reaction, increasing sensitivity even in low-volume reactions.
Selection Guide of One-Step Kit (probe based):
Cat. No. | V5001/V5002 | V5005/V5006 | V5005L/V5006L | V5007/V5008 | V5007L/V5008L | V5009/V5010 | V5011 | V5012 | |
Product Name | One-step Probe RT-qPCR Kit | DSPathTM 4X One-Step Multiplex Master Mix | DSPathTM 4X One-Step Multiplex Master Mix (Lyophilized) | 2X One Step Prime RT-qPCR Mix | 2X One Step Prime RT-qPCR Mix (Lyophilized) | One-step Probe RT-qPCR Kit V2 | One-step Probe RT-qPCR Kit V3 | Super 4X One-Step Multiplex Master Mix | |
Mixture Format | Enzyme Mix+2X Buffer Mix | 4X All-in Mix | 4X All-in Lyophilized Powder | 2X All-in Mix | 2X All-in Lyophilized Powder | Enzyme Mix+2X Buffer Mix | Enzyme Mix+5X Buffer Mix | 4X All-in Mix | |
Mode/Time | S: ~120 min | F: ~60 min S: ~120 min | F: ~60 min S: ~120 min | F: ~60 min S: ~120 min | F: ~60 min S: ~120 min | F: ~60 min S: ~80 min | F: ~45 min S: ~80 min | F: ~70 min S: ~130 min | |
RT TEMP | 50~55°C | 48~55°C | 48~55°C | 48~55°C | 48~55°C | 50~55°C | 50~55°C | 48~55°C | |
PCR Enzyme | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | |
Heat-labile UDG | + | + | + | + | + | + | + | + | |
Sensitivity | Very high | Very high | Very high | Very high | Very high | Ultra high | Ultra high | Ultra high |
S: standard mode; F: fast mode
V5012, V5009, and V5011 are recommended
Application
• Probe gene expression analysis
• Probe Low-copy gene detection
• Probe microarray validation
• Probe gene knockdown validation
Features
• support 10-plex amplification with high specificity
• Reverse transcription at 55 °C to accommodate complex RNA templates
• Contains heat-labile UDG to reduce false-positive contamination
• High sensitivity for detection of low-abundance genes
• High stability
• 4X formation, high concentration
• Saves time by supporting fast program
Components
Component | V5012-A (200rxns) rxns)rxns) | V5012-B (5000 rxns) |
Super 4X One-Step Multiplex Master Mix | 1 ml × 1 | 25 ml × 1 |
Storage Condition
This reagent should be kept at -15~-25°C.
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Guangzhou Dongsheng Biotech Co., Ltd. Web: www.gdsbious.com Email: order@gdsbio.com WhatsApp/Wechat: +86 19128993865 TEL: 86-020-31600213 ADD: Room 305, Building A, No. 179, Guangpu East Road, Guangzhou 510760, China | Recommend |
