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V5001 One-step Probe RT-qPCR Kit

Products & Applications > Molecular Research Reagents > V5001 One-step Probe RT-qPCR Kit

V5001 One-step Probe RT-qPCR Kit

Price: $200.00~4000.00
V5001(200 rxns) V5002(5000 rxns)

Product Introduction

The One-step Probe RT-qPCR Kit is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55°C, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Integrating the superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0.1 pg total RNA or <10 copies of RNA template. The kit is available as a convenient Master Mix. 2 × One-step U+ Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity detection systems with fluorescent labeled probes such as TaqMan®. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.

Selection Guide of One-Step Kit (probe based):

Cat. No.


V5001/V5002

V5005/V5006

V5005L/V5006L

V5007/V5008

V5007L/V5008L

V5009/V5010

V5011

Product Name


One-step Probe RT-qPCR Kit

DSPathTM 4X One-Step Multiplex Master Mix

DSPathTM 4X One-Step Multiplex Master Mix (Lyophilized)

2X One Step Prime RT-qPCR Mix

2X One Step Prime RT-qPCR Mix (Lyophilized)

One-step Probe RT-qPCR Kit V2

One-step Probe RT-qPCR Kit V3

Mixture Format


Enzyme Mix+2X Buffer Mix

4X All-in Mix

4X All-in Lyophilized Powder

2X All-in Mix

2X All-in Lyophilized Powder

Enzyme Mix+2X Buffer Mix

Enzyme Mix+5X Buffer Mix

Mode/Time


S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~80 min

F: ~45 min S: ~80 min

RT TEMP


50~55°C

48~55°C

48~55°C

48~55°C

48~55°C

50~55°C

50~55°C

PCR Enzyme


Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Heat-labile UDG


+

+

+

+

+

+

+

Sensitivity


Very high

Very high

Very high

Very high

Very high

Ultra high

Ultra high

S: standard mode; F: fast mode

V5005, V5009, and V5011 are recommended

 

Application

Probe gene expression analysis

Probe Low-copy gene detection

Probe microarray validation

Probe gene knockdown validation

Features

One-step RT-qPCR

This kit is suitable for fluorescence quantification by probe method

This kit is compatible with many real-time systems

Hot-start technology brings high specificity and reproducible amplification

dUTP/UDG system, effectively prevent PCR product contamination

Components

Component

V5001

(200 rxns)

V5002

(5000 rxns)

2X One-step U+ Mix

760 μl × 4 

38.4 ml × 2

One-step U+ Enzyme Mix

308 μl  

7.7 ml

RNase-free ddH2O

800 μl × 4 

76 ml

50X ROX Reference Dye 1 

120 μl

1 ml × 3

50X ROX Reference Dye 2

120 μl

1 ml × 3

Storage Condition

Store at -20°C


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