PicoGreen dsDNA Quantitation Reagent P6021

PicoGreen dsDNA Quantitation Reagent

Cat. No. / Spec.: P6021 / 1 mL

Description

PicoGreen is a highly sensitive fluorescence detection reagent specifically designed for the quantitation analysis of double-stranded DNA (dsDNA). It is widely used in molecular biology research and bioproduct testing due to its excellent sensitivity, specificity, and convenience. PicoGreen only emits fluorescence upon binding to dsDNA, and the fluorescence intensity is proportional to the DNA concentration, making it an ideal choice for quantifying dsDNA.

Components

Storage

Store at 4°C away from light.

Parameters

Excitation/Emission Wavelength: Ex/Em = 480/520 nm (when bound to dsDNA)

Application

Quantitation of PCR products, genomic DNA quantitation, determination of dsDNA in complex mixtures, viral DNA quantitation, etc.

Features

High Sensitivity: Can detect dsDNA in the range of 25 pg/mL to 1000 ng/mL with a good linear relationship (R²>0.99).

Easy Operation: The quantitation method is simple and quick, easy to perform in the laboratory.

Strong Anti-interference Ability: Basically unaffected by single-stranded DNA (ssDNA) and RNA, can tolerate certain concentrations of salts, urea, ethanol, chloroform, detergents, proteins, and other interferences.

Wide Linear Range: The fluorescence response has a broad linear range, covering multiple orders of magnitude.