【Product Name】
GDSNext RNA First Strand Synthesis Module
【Cat. No./Spec.】
K020-A/24 rxns; K020-B/96 rxns
【Product Description】
The GDSNext RNA First Strand Transcription Module is an optimized RNA first-strand transcription module that can transcribe a wide range of input RNA into cDNA using random primers.
This module can be used in conjunction with the GDSNext Directional RNA Second Strand Synthesis Module (# K021) for specific RNA second-strand synthesis or the GDSNext Non-Directional RNA Second Strand Synthesis Module (# K022) for non-specific RNA second-strand synthesis. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion and enable high-yield preparation of high-quality libraries from 10 ng to 1 µg of total RNA.
【Components】
Component | K020-A (24 rxns) | K020-B (96 rxns) |
GDSNext First Strand Synthesis Enzyme Mix | 192 µL | 768 µL |
GDSNext First Strand Synthesis Reaction Buffer | 48 µL | 192 µL |
Random Primers | 48 µL | 192 µL |
GDSNext Strand Specificity Reagent | 192 µL | 768 µL |
【Storage Condition & Shelf Life】
All reagents should be stored at -20°C. The product is valid for 18 months.
【Application】
For the synthesis of the first-strand cDNA of RNA in NGS library preparation.