tel.png Tel: +86 20 31600213    mail.png Sales EMail: order@gdsbio.com      

XhoI E1029-A E1029-B

XhoI E1029-A E1029-B

Price: $50.50~149.00
E1029-A/400 rxns E1029-B/1,200 rxns

Xho


Cat. No./Spec.: E1029-A/400 rxns, E1029-B/1,200 rxns

Recognition site:

5'   C ↓ T   C   G   A   G     3'  

3'   G   A   G   C   T ↑ C     5'  

 

Product Description 

The Xho restriction endonuclease recognizes the C^TCGAG site and achieves optimal cutting efficiency within 515 minutes at 37°C using the universal buffer. Isoschizomers: PaeR7, Sfr274, Sla, Str, Tli.

GDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.

The universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.

The Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.

 

Components

Component

E1029-A 

E1029-B

Xho

400 µL

1.2 mL

10X Digest Buffer

1 mL

1 mL × 3

10X Green Buffer

1 mL

1 mL × 3

 

Storage Condition & Shelf Life

Store at -20°C.

 

Features

- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.

- 100% buffer compatibility with downstream applications.

- Enzymatic digestion can be completed within 515 minutes.

- Direct loading onto a gel.

- No star activity.

 

Scope of Application 

- Molecular cloning

- Restriction site mapping

- Genotyping

- Southern blotting

- Restriction fragment length polymorphism (RFLP)

- SNP (Single Nucleotide Polymorphism) analysis


Name
Phone
Email
Corporate name
Address
Leaving Message*
 
Verification code
Submit