S1 Nuclease E1017-A

S1 Nuclease

Cat. No./Spec.: E1017-A/10,000 U 

Concentration: 100 U/µL

Product Description 

S1 nuclease can degrade single-stranded nucleic acids, releasing 5'-phosphorylated mononucleotides or oligonucleotides. Its activity on DNA is five times that on RNA. S1 nuclease can also cleave double-stranded DNA (dsDNA) at single-stranded regions caused by nicks, gaps, mismatches, or loops. S1 nuclease exhibits 3'-phosphomonoesterase activity.

This enzyme is a glycoprotein with a carbohydrate content of 18%.

Components

Storage Condition & Shelf Life

Store at -20°C.

Source

Aspergillus oryzae strain.

Unit Definition

A unit is defined as the amount of enzyme required to produce 1 µg of acid-soluble deoxyribonucleotides in 1 minute at 37°C.

Enzyme activity is determined in the following mixture: 30 mM sodium acetate (pH 4.5), 50 mM NaCl, 0.1 mM ZnCl₂, 5% (v/v) glycerol, and 800 µg/mL heat-denatured calf thymus DNA.

Scope of Application 

- Removal of single-stranded overhangs from DNA fragments

- Localization of S1 transcripts

- Cleavage of hairpin loops

- In conjunction with Exonuclease III, creating unidirectional deletions within DNA fragments

Inhibition and Inactivation

- Inhibitors: metal chelating agents, PPi (inorganic pyrophosphate), Pi (inorganic phosphate), 5'-ribonucleotides, and deoxyribonucleotides.

- Inactivate by heating at 70°C for 10 minutes in the presence of EDTA.