Product Introduction
PCR MixII is an upgraded Hotstart version of PCR Mix, offering better sensitivity, specificity, and reaction speed compared to regular PCR. PCR MixII is a 2X concentrated PCR amplification premix solution containing Hotstart Taq DNA polymerase, dNTPs, buffer, and other essential components for PCR amplification (excluding template and primers). When using it, simply add the template and primers to the amplification system to perform PCR, which greatly simplifies the operation process, reduces operation time, and minimizes contamination. The system also includes an electrophoresis indicator, allowing the PCR amplification products to be directly detected by electrophoresis. The amplified products have a 3'-dA overhang, which can be directly used for TA cloning.
Applications
• Routine PCR
• Generation of PCR products for TA cloning
Product Advantages
• Higher sensitivity and specificity.
• Extremely stable with minimal batch-to-batch variation.
• Suitable for a wide range of PCR applications.
• High cost-performance ratio.
• Pre-mixed with indicator dye, allowing direct electrophoresis of products.
Storage
Store at -20°C for 2 years.