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P1081 HS Taq DNA Polymerase

Products & Applications > High Specificity PCR > P1081 HS Taq DNA Polymerase

P1081 HS Taq DNA Polymerase

Price: $3.50~176.00
P1081(250U) P1082(500 U) P1083(1,000 U) P1084(18,000 U)

Product Introduction

HS Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. HS Taq DNA Polymerase can amplify DNA target up to 5 kb. The elongation velocity is 2kb/min. It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product. All components of the HS PCR Buffer are at optimal concentration for efficient amplification. It contributes to highly specific incorporation of primer and template.

Application

• PCR amplification of DNA fragments as long as 5 kb

• DNA labeling

• DNA sequencing

• PCR for cloning

Features

High specificity (not hotstart), 2kb/min, generates 3'-dA overhangs

Components

Component

P1081

(250U)

P1082

(500U)

P1083

(1000U)

P1084

(18000U)

HS Taq DNA Polymerase (5U/μl)

50 μl

100 μl

200 μl 

100 μl × 36

10× HS PCR Buffer (Mg2+ Plus) *

1.25 ml

1.25 ml

1.25 ml × 2

1.25 ml × 36

6× Loading Buffer

1 ml

1 ml

1 ml

-

* Mg2+ Free version is also available. 

Storage Condition

Store at -20°C


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