20bp Ladder DNA Marker M1021/M1022

CLASSIC DNA MARKER of GDSBIO

Product Name

20bp Ladder

Cat. No./Spec.

M1021/50 μg, M1022/50 μg×5

Description

20bp Ladder is an ideal DNA Ladder for determining the size of double-stranded DNA (dsDNA) from 60 to 300 base pairs (bp) in high percentage agarose or non-denaturing polyacrylamide gels. The ladder consists of 13 linear dsDNA fragments. It is a 20 bp step ladder, especially useful for determining 20 bp differences between DNA fragments. The 100bp and 200bp fragments are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. This ladder is pre-mixed with loading dye and is ready to use.

Application

• agarose gel electrophoresis, AGE

• Non-denaturing polyacrylamide gel electrophoresis (PAGE)

• pulsed field gel electrophoresis, PFGE

Features

• Clear and bright bands for accurate molecular weight determination

• Stable quality allows for use at room temperature

• Multiple ranges available with 23 classic series and 18 LD series options to suit different gel dyes

• Two Marker series are suitable for different types of gel dyes, and the strips are not easily deformed

Recommended Amount

1 µl/Lane in non-denaturing polyacrylamide gel

3-5 µl/Lane in agarose gel

Recommended Electrophoresis Condition

1 µl/Lane, 20 cm 10% non-denaturing Polyacrylamide Gel, 1×TBE, 8 V/cm, 3h.

Bands (bp)

60、80、100、120、140、160、180、200、220、240、260、280、300.

Concentration

Total concentration, 128 ng/µl

Typical bands, 20 ng/µl

Other bands, 8 ng/µl

Storage Condition

Stable for 3 months at RT, for long-time storage, please store at -20 ̊C. 

Components