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Proteinase K (recombinant), PCR grade E1019-A E1019-B

Products & Applications > Tool Enzyme & Protein Ladder > Proteinase K (recombinant), PCR grade E1019-A E1019-B

Proteinase K (recombinant), PCR grade E1019-A E1019-B

Price: $47.50~215.00
E1019-A/1ml E1019-B/1ml x 5

Proteinase K (recombinant), PCR grade


Cat. No./Spec.: E1019-A/1 mL; E1019-B/1 mL × 5 

Concentration:  600 U/mL (~20 mg/mL)

 

Product Description 

Proteinase K is an endopeptidase capable of hydrolyzing peptide bonds at the carboxyl terminus of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K belongs to the family of serine proteases. This enzyme can minimally hydrolyze tetra-peptides.

 

Components

Component

E1019-A 

E1019-B

Proteinase K (recombinant), PCR grade

1 mL

1 mL×5

 

Storage Condition & Shelf Life

Store at -20°C.

 

Source

Recombinant Pichia pastoris strain containing a cloned gene from Tritirachium album.

 

Unit Definition

A unit is defined as the amount of enzyme required to release an amount of Folin-positive amino acids and peptides equivalent to 1 µmol of tyrosine from denatured hemoglobin in 1 minute at 37°C.

Enzyme activity is determined in the following mixture: 0.08M potassium phosphate (pH 7.5), 5M urea, 4mM NaCl, 3mM CaCl2, and 16.7 mg/mL hemoglobin.

 

Features

- Ready-to-use solution

- Compatible with a wide range of reaction conditions

Scope of Application 

- Isolate genomic DNA from mouse tails.

- Isolate genomic DNA from cultured cells.

- Eliminate DNase and RNase during the isolation of DNA and RNA from tissues or cell lines.

- Determine the location of enzymes.

- Improve the cloning efficiency of PCR products.

 


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