Product Introduction
Hotstart KnTaq Polymerase is an N-truncated Taq DNA polymerase with a stable hot-start system and no 5'-3' exonuclease activity. It is optimized for excellent differentiation of error extensions, and can be used for SNP analysis, genotyping and allele-specific PCR.
Application
• SNP analysis and genotyping
• Allele-specific PCR
• Multiplexing
• Arbitrarily primed PCR
• Formulation of dried-down amplification reagents
Features
• Thermostable: half-life over 40 min at 95°C incubation
• Generates 3'-dA overhangs PCR products
Components
Component | P1221 (250U) | P1222 (1000U) | P1223 (5000U) | P1224 (50000U) |
Hotstart KnTaq Polymerase (5U/μl) | 50 μl | 200 μl | 1 ml | 1 ml × 10 |
10× Taq Buffer (Mg2+ Plus)* | 1.25 ml | 1.25 ml × 2 | 1.25 ml × 10 | 50 ml × 10 |
* Mg2+ Free version is also available.
Storage Condition
Store at -20°C