Product Introduction
Super Hotstart Taq Polymerase is a hot-start polymerase with dual-antibody modification, which brings higher specificity by reducing non-specific products as the enzyme activity is temperature-dependent and is inhibited at room temperature. Its initial-denaturation time can be reduced to 1 minute. Super Hotstart Taq provides excellent specificity, sensitivity and yield. The reaction system can be prepared at room temperature. The amplification length and speed can reach to 5 kb (simple template) and 2min/kb (simple template up to 20s/kb) separately. Super Hotstart Taq has 5’-3’ polymerase activity, but no 3’-5’ exonuclease activity. The products of Super Hotstart Taq have overhanged dA at 3’-end.
Application
• High-specificity amplification
• Multiplex PCR
• IVD product development and testing
Features
• High specificity
• hotstart with dual-antibody modification
• Elongation speed: 2min/kb
• generates 3'-dA overhangs
Components
Component | P1201 (250U) | P1202 (1000U) | P1203 (5000U) | P1204 (50000U) |
Super Hotstart Taq Polymerase (5U/μl) | 50 μl | 200 μl | 1 ml | 1 ml × 10 |
10× Hotstart Buffer (Mg2+ Plus)* | 1.25 ml | 1.25 ml × 2 | 1.25 ml × 10 | 50 ml × 10 |
* Mg2+ Free version is also available.
Storage Condition
Store at -20°C