Product Introduction
The NGS Multiplex PCR Master Mix contains all of the components for NGS multiplex PCR (except for primers and templates) in a single tube, including chemically modified Hotstart DNA Polymerase. With the low error rates and high specificity, the PCR products can be used for NGS sequencing. The mix also includes the GC Enhancer for difficult-to-amplify templates, especially for templates with high GC content.
Application
This product is suitable for PCR amplification of NGS libraries.
Features
Multiplex PCR with high fidelity
Hotstart DNA Polymerase with high specificity
Components
Component | NM1001 (40 rxns) | NM1002 (400 rxns) | NM1003 (2000 rxns) |
NGS Multiplex PCR Master Mix, 2X | 1 mL × 1 | 10 mL × 1 | 10 mL × 5 |
GC Enhancer | 0.25 mL × 1 | 1 mL × 2 | 10 mL × 1 |
Storage Condition
Store unopened at –15°C to –25°C until the expiration date on the label. After opening, the master mix may be stored at –15°C to –25°C until the expiration date on the label, or at 4°C for up to 30 days.
The GC Enhancer must be kept at –15°C to –25°C.