T4 DNA Polymerase K011-A K011-B K011-C

Product Introduction

In the presence of templates and primers, T4 DNA Polymerase catalyzes DNA synthesis in the 5 →3 direction. This enzyme also has the activity of 3 →5 exonuclease which is stronger than E. coli DNA polymerase I. Unlike DNA polymerase I, T4 DNA Polymerase does not have 5 →3 exonuclease activity.

Application

1. Removal of 3’ overhangs to form blunt ends; 

2. Fill-in of 5’ overhangs to form blunt ends; 

3. Labeling DNA probe synthesis through displacement reaction; 

4. Synthesis of the second strand during site-directed mutagenesis; 

5. PCR product cloning without relying on ligation reactions.

Features

5 ́→3 ́ polymerase activity

3 ́→5 ́ exonuclease activity

No 5 ́→3 ́ exonuclease activity

Experimental Cases

无

Components

Storage Condition

All reagents should be stored at -20°C.