Product Introduction
Klenow Fragment (3´→ 5´ exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5´→ 3´ exonuclease activity and has mutations which abolish the 3´→ 5´ exonuclease activity.
Application
1.Generates probes using random primers
2.Random priming labeling
3.dA-tailing at 3' end
4.Second strand cDNA synthesis
Features
Moderate strand displacement activity
Experimental Cases
无
Components
Component | K010-A (100U) | K010-B (200U) | K010-C (1,000U) |
Klenow Fragment(3´→5´ exo-)(5U/µL) | 20 µL | 40 µL | 200 µL |
10X Klenow Buffer | 1.25 mL | 1.25 mL | 1.25 mL |
Storage Condition
All reagents should be stored at -20°C.